Plasmid

Part:BBa_K3183050:Design

Designed by: Natasha Cooke   Group: iGEM19_Oxford   (2019-10-16)


pTRKH3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 7723
    Illegal suffix found in sequence at 1
    Illegal EcoRI site found at 5869
    Illegal XbaI site found at 1026
    Illegal XbaI site found at 7293
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5869
    Illegal EcoRI site found at 7723
    Illegal NheI site found at 5318
    Illegal NheI site found at 6451
    Illegal NheI site found at 7592
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 7729
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5869
    Illegal EcoRI site found at 7723
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 7723
    Illegal suffix found in sequence at 2
    Illegal EcoRI site found at 5869
    Illegal XbaI site found at 1026
    Illegal XbaI site found at 7293
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 7723
    Illegal EcoRI site found at 5869
    Illegal XbaI site found at 1026
    Illegal XbaI site found at 7293
    Illegal XbaI site found at 7738
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 360
    Illegal NgoMIV site found at 520
    Illegal NgoMIV site found at 874
    Illegal AgeI site found at 6537
    Illegal AgeI site found at 6861
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None as this is a standard plasmid for Lactobacterial work.


Source

Artificial cloning vectors, obtained from Addgene

References